The present invention relates to a method for reducing the amount of acidic species and/or increasing the amount of basic species in a composition comprising a recombinant protein or for increasing the size of mammalian cells expressing a recombinant protein or for increasing the specific production rate of mammalian cells expressing a recombinant protein to simplify subsequent clarification and purification of the recombinant protein, said method comprising culturing mammalian cells expressing said recombinant protein in a cell culture medium comprising citrulline. The method according to claim 26, wherein the second temperature is from 25° C. The method according to claim 19, wherein the temperature is changed from a first temperature to a second temperature during the culturing.Ģ7. The method according to claim 19, wherein the citrulline is added to the cell culture medium.Ģ6. The method according to claim 23, wherein each of the basal medium and the feed medium comprises citrulline.Ģ5. The method according to claim 19, wherein the cells are cultured in fed-batch mode using first a basal medium and then a feed medium.Ģ4. The method according to claim 19, wherein no additional lysine is added to the cell culture medium.Ģ3.
![dessiner molecule mestrenova dessiner molecule mestrenova](https://image.slidesharecdn.com/attributionetsimulationautomatiqueparmnova-190621141410/95/attribution-et-simulation-automatique-par-mnova-5-638.jpg)
The method according to claim 19, wherein the cell culture medium further comprises 5 to 80 mM sucrose.Ģ2. The method according to claim 19, wherein the composition has a reduced amount of acidic species of the antibody compared to a composition produced by culturing said cells in a cell culture medium not comprising 2 to 30 g/l citrulline.Ģ1. A method of producing a composition comprising a recombinant antibody in Chinese Hamster Ovary cells expressing said antibody, the method comprising: (a) culturing said cells in a cell culture medium comprising 2 to 30 g/l citrulline and (b) obtaining the composition comprising the antibody from the cell culture medium.Ģ0. The method according to claim 1, wherein the eukaryotic cells are mammalian cells.ġ9. The method according to claim 1, wherein the recombinant protein is a recombinant antibody.ġ7. The method according to claim 14, wherein the second temperature is from 25° C. The method according to claim 1, wherein the temperature is changed from a first temperature to a second temperature during the culturing.ġ5. The method according to claim 1, wherein no additional lysine is added to the cell culture medium.ġ4.
![dessiner molecule mestrenova dessiner molecule mestrenova](https://www.superprof.fr/ressources/wp-content/uploads/2017/03/molecules.jpg)
![dessiner molecule mestrenova dessiner molecule mestrenova](https://www.epfl.ch/campus/art-culture/museum-exhibitions/archizoom/wp-content/uploads/2018/11/Gachet_affiche.jpg)
The method according to claim 11, wherein each of the basal medium and the feed medium comprises citrulline.ġ3.
![dessiner molecule mestrenova dessiner molecule mestrenova](https://actu.epfl.ch/image/112084/11296x728.jpg)
The method according to claim 1, wherein the cells are cultured in fed-batch mode using first a basal medium and then a feed medium.ġ2. The method according to claim 1, wherein the citrulline is added to the cell culture medium.ġ1. The method according to claim 1, wherein the cell culture medium comprises 2 to 30 g/l citrulline.ħ. A method for increasing the specific production rate of eukaryotic cells expressing a recombinant protein, said method comprising culturing said cells expressing said recombinant protein in a cell culture medium comprising citrulline and/or sucrose.Ħ. A method for reducing the amount of acidic species and/or increasing the amount of basic species in a composition comprising a recombinant protein, said method comprising culturing eukaryotic cells expressing said recombinant protein in a cell culture medium comprising citrulline.Ĥ.